HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) is the fourth generation of the HiScript series for synthesizing first strand cDNA using Total RNA or Poly A+RNA as templates. Compared to previous versions, the HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) further enhances reverse transcription efficiency, including stronger extension ability, faster reaction speed and higher tolerance to inhibitors, especially suitable for downstream qPCR quantification and long fragment cDNA amplification. This product premixes HiScript IV RTase, RNase inhibitor, dNTP Mix, etc., and only appropriate reverse transcription primers need to be added according to downstream experiments. The kit provides a genomic DNA elimination module, where the 5 × gDNA wiper Mix in the kit can quickly remove genomic DNA contamination under conditions of 42°Cfor 2 min, ensuring reliable and authentic experimental data.
