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SKU:EN401-01

DNase I, RNase-free (1 U/µl)

DNase I, RNase-free (1 U/µl)

Regular price 27.800 Ft
Regular price Sale price 27.800 Ft
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Deoxyribonuclease I (DNase I) is an endonuclease that digests single- or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a single deoxynucleotide or single- or double-stranded oligodeoxynucleotide with 5'-phosphorylated and 3'-hydroxylated ends. The activity of DNase I depends on Ca2+, and DNase I can also be activated by divalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at any site on either strand, and in the presence of Mn2+, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with one or two nucleotide overhangs.

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Store at -30 ~ -15°C and transport at ≤0°C.

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