This kit is applicable for extracting bacterial culture that has been cultured overnight in a volume of 15 - 50 ml. It uses an optimized alkaline lysis method to lyse cells and utilizes a unique silica membrane adsorption technique along with special Buffer ERB and Buffer ERW solutions to specifically bind plasmids and effectively remove impurities such as endotoxins, proteins, and genomic DNA. Additionally, the unique P2 solution with indicator can indicate the completeness of lysis and neutralization through color changes, ensuring extraction quality and enabling visual operation. The extracted plasmid DNA has extremely low residual endotoxin levels, making it suitable for various cell transfection and routine operations, including restriction endonuclease digestion, PCR, sequencing, ligation, and other experiments.
Shipping and Storage
BOX 1: RNase A Solution. Store at 2 ~ 8°C and ship on ice pack. \n
BOX 2: Other components. Store at 15 ~ 25°C and ship at room temperature. \n
The RNaseA can be stored at room temperature for one week, and at 4℃ for one month.\n
