FastPure Plasmid Mini Kit purifies 1 - 5 ml of cultured bacteria and lyses the cells by an optimized SDS-alkaline method. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, and binds plasmid DNA in a highly efficient manner under high-salt and low-pH conditions and largely removes proteins, genomic DNA, RNA, and other impurities. In the end, pure plasmid DNA is eluted from the silica membrane with a low-salt, high-pH elution buffer. The kit recovers up to 35 µg of high-copy plasmid DNA per isolation procedure. The purified plasmid DNA is ready for use in biological experiments such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of common passage cells.
Shipping and Storage
Store at 15 ~ 25°C and transport at room temperature.
If ambient temperatures often exceed 25°C, we suggest storing RNase A at 2 ~ 8°C. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2 ~ 8°C.
