One Step qRT-PCR SYBR Green Kit is specially designed for qPCR detection using RNA (eg, RNA virus) as templates. Reverse transcription and qPCR can be finished in one tube in the presence of Gene Specific Primers (GSP). There are no additional opening/pipetting operations required, which greatly increases assay throughput and reduces the risk of contamination. Intergrating the superior performance of HiScript II Reverse Transcriptase and Champagne Taq plus DNA Polymerase with the optimized buffer, the lowest RNA template for detection of this kit is 0.1 pg. The specificity-enhancing factor existing in the buffer can effectively reduce the formation of primary dimers and improve the specificity of the product. The kit is provided as the formation of master mix, which is convenient to use.
Shipping and Storage
Store at -30～-15℃ for up to 12 months, and transport at ≤0℃．
2×One Step SYBR Green Mix should be stored at -30～-15℃ away from light.
