SKU:R312-01

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

123.900 Ft

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Size 50 rxns (20 µl/rxn) 100 rxns (20 µl/rxn)

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DESCRIPTION

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), including a new generation of reverse transcriptase HiScript III Reverse Transcriptase and Buffer optimized for reverse transcription, further improving the efficiency of 1st strand synthesis. The 5 × gDNA wiper Mix in the kit can quickly remove genomic DNA contamination within 2 min, which makes the results more reliable and simplifies the process of qPCR primer design without the need of spanning an exon-exon junction. The kit contains single-component reverse transcription primers Oligo (dT) ₂₀ VN and Random hexamers, which allows users to choose primers flexibly for subsequent experiments as required. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.

Shipping and Storage

Store at -30 ~ -15℃ for up to 18 months, and transport at ≤0℃.

10 × RT Mix contains a high concentration of DTT, which may precipitate at low temperatures. Please restore to room temperature, shake gently and mix thoroughly, wait to dissolve the precipitation again before use.