HiScript III One Step qRT-PCR Probe Kit is specially designed for qPCR that directly uses RNA (eg virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step Mix contains an optimized buffer and dNTP/dUTP Mix, and is suitable for high-specificity detection systems based on fluorescence labeled probes (eg TaqMan).
