The Ribo-off rRNA Depletion Kit V2 (Bacteria) is designed to remove ribosomal RNA (rRNA) from the total RNA of gram-positive and gram-negative bacteria. This kit is compatible with an initial template input of 0.01 - 1 μg. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, and other steps for the rRNAs (including 16S and 23S rRNA) to be depleted while retaining mRNA and other non-coding RNA. This kit is applicable to both intact and partially degraded RNA samples, and the resulting RNA can be used for the analysis of mRNA and non-coding RNA (such as LncRNA). The optimized reaction system enhances the depletion effect and shortens the workflow time.
