Long-range amplification: gDNA≤40 kb, Plasmid≤30 kb, λDNA≤17.8 kb and cDNA≤15 kb.
Multiplex amplification: Compatible with 12-plex PCR of 300 bp-15 kb fragments.
High success rate: Robust amplification of complex systems (GC-rich/AT-rich, lysis products, plaque/bacterial suspension, whole blood/cell/leaf direct amplification, etc.)
Simple and fast: Extension rate of 15-30 sec/kb; Include tracking dye for direct loading of PCR products on gels.
DESCRIPTION
KeyPo SE DNA Polymerase is a new generation of high-fidelity DNA polymerase based on the BioSmart platform, which features an ultra-high amplification success rate and rapid amplification of 15 sec/kb. With the latest hot start technology and optimized buffer system, it can perform highly specific single-plex PCR for up to 40 kb, and highly uniform multiplex PCR within the range of 300 bp - 15 kb, which minimizes GC bias and allows for more uniform amplification of various regions of genomic DNA and cDNA. The 2 × KeyPo SE Master Mix (Dye Plus) is a premix containing KeyPo SE DNA Polymerase, dNTPs, Mg²⁺, and other necessary components for PCR reactions. It only requires the addition of primers and templates for amplification, thereby reducing pipetting operations and improving detection on throughput and reproducibility of results. It is applicable for regular templates, crude samples, uracil-containing DNA, long fragments (40 kb), and GC-rich systems (primers or templates). This product contains tracking dyes, and amplification products can be directly loaded for gel electrophoresis after the reaction. The amplification products are blunt-ended, which are subsequently applicable for the ClonExpress Ultra One Step Cloning Kit V2 (Vazyme #C116) and the Ultra-Universal TOPO Cloning Kit (Vazyme #C603).
