During apoptosis, intracellular specific endonucleases are activated, chromatin DNA is specifically cleaved between nucleosomes, and DNA is degraded into integer multiples of about 180 - 200 bp fragments. The 3'-hydroxyl (3'-OH) end generated by DNA fragmentation can bind to fluorescein-12-deoxyuridine triphosphate (FITC-12-dUTP) under the action of terminal deoxynu-cleotidyl transferase (TdT). FITC-12-dUTP-labeled DNA can be directly observed by fluorescence microscopy or quantitatively analyzed by flow cytometry to reflect the level of apoptosis. The BrightGreen Labeling Mix contains FITC-12-dUTP and Bright factors. This unique small molecule compound can non-covalently bind to FITC, enhance its stability and amplify its signal, resulting in brighter labeled fluorescence and stronger anti-quanching ability.
Shipping and Storage
Store at -20℃ for up to 12 months; Store BrightRed Labeling Mix at -20℃ in the dark.
